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Construction of plasmids carrying the cI gene of bacteriophage lambda.

By techniques of recombination in vitro, we have constructed a plasmid bearing the repressor gene (cI) of bacteriophage lambda fused to the promoter of the lac operon. Strains carrying this plasmid overproduce lambda repressor. This functional cI gene was reconstituted by joining DNA fragments beari...

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Detalles Bibliográficos
Publicado en:Proc Natl Acad Sci U S A
Autores principales: Backman, K, Ptashne, M, Gilbert, W
Formato: Artigo
Lenguaje:Inglês
Publicado: National Academy of Sciences 1976
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Acceso en línea:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC431373/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/1069307/
https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.73.11.4174
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