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Label-free detection of kanamycin based on a G-quadruplex DNA aptamer-based fluorescent intercalator displacement assay

This work was the first to report that the kanamycin-binding DNA aptamer (5′-TGG GGG TTG AGG CTA AGC CGA-3′) can form stable parallel G-quadruplex DNA (G4-DNA) structures by themselves and that this phenomenon can be verified by nondenaturing polyacrylamide gel electrophoresis and circular dichroism...

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Detalhes bibliográficos
Publicado no:Sci Rep
Main Authors: Xing, Yun-Peng, Liu, Chun, Zhou, Xiao-Hong, Shi, Han-Chang
Formato: Artigo
Idioma:Inglês
Publicado em: Nature Publishing Group 2015
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC4311242/
https://ncbi.nlm.nih.gov/pubmed/25634469
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/srep08125
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