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An Enzyme- and Label-Free Fluorescence Aptasensor for Detection of Thrombin Based on Graphene Oxide and G-Quadruplex

An enzyme- and label-free aptamer-based assay is described for the determination of thrombin. A DNA strand (S) consisting of two parts was designed, where the first (Sa) is the thrombin-binding aptamer and the second (Se) is a G-quadruplex. In the absence of thrombin, Sa is readily adsorbed by graph...

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Detalhes bibliográficos
Publicado no:Sensors (Basel)
Main Authors: Wei, Yani, Wang, Luhui, Zhang, Yingying, Dong, Yafei
Formato: Artigo
Idioma:Inglês
Publicado em: MDPI 2019
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC6832557/
https://ncbi.nlm.nih.gov/pubmed/31614837
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3390/s19204424
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