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Isolation of a set of hybrid lac repressors made in vitro between normal lac repressor and its homogeneous tryptic core.
Lactose repressor can be renatured from 8 M guanidine-HCl solution. The renatured repressor is tetrameric and shows DNA binding activity. Thus it becomes possible to obtain hybrid tetramers in vitro between normal repressor and repressor defective in DNA binding by simultaneous denaturation and rena...
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| Main Authors: | , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
1976
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC430944/ https://ncbi.nlm.nih.gov/pubmed/787984 |
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