Beneficial Effect of Sugar Osmolytes on the Refolding of Guanidine Hydrochloride-Denatured Trehalose-6-phosphate Hydrolase from Bacillus licheniformis

The influence of three sugar osmolytes on the refolding of guanidine hydrochloride- (GdnHCl-) denatured trehalose-6-phosphate hydrolase of Bacillus licheniformis (BlTreA) was studied by circular dichroism (CD) spectra, fluorescence emission spectra, and the recovery of enzymatic activity. These expe...

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Detalhes bibliográficos
Publicado no:Biomed Res Int
Main Authors: Chen, Jiau-Hua, Chi, Meng-Chun, Lin, Min-Guan, Lin, Long-Liu, Wang, Tzu-Fan
Formato: Artigo
Idioma:Inglês
Publicado em: Hindawi Publishing Corporation 2015
Assuntos:
Research Article
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC4309298/
https://ncbi.nlm.nih.gov/pubmed/25667926
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1155/2015/806847
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