Targeting mammalian organelles with internalizing phage (iPhage) libraries

Techniques largely used for protein interaction studies and discovery of intracellular receptors, such as affinity capture complex purification and yeast two-hybrid, may produce inaccurate datasets due to protein insolubility, transient or weak protein interactions, or irrelevant intracellular conte...

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Détails bibliographiques
Publié dans:Nat Protoc
Auteurs principaux: Rangel, Roberto, Dobroff, Andrey S., Guzman-Rojas, Liliana, Salmeron, Carolina C., Gelovani, Juri G., Sidman, Richard L., Pasqualini, Renata, Arap, Wadih
Format: Artigo
Langue:Inglês
Publié: 2013
Sujets:
Article
Accès en ligne:https://ncbi.nlm.nih.gov/pmc/articles/PMC4309278/
https://ncbi.nlm.nih.gov/pubmed/24030441
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/nprot.2013.119
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