Selecting Open Reading Frames From DNA

We describe a method to select DNA encoding functional open reading frames (ORFs) from noncoding DNA within the context of a specific vector. Phage display has been used as an example, but any system requiring DNA encoding protein fragments, for example, the yeast two-hybrid system, could be used. B...

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Bibliographic Details
Main Authors: Zacchi, Paola, Sblattero, Daniele, Florian, Fiorella, Marzari, Roberto, Bradbury, Andrew R.M.
Format: Artigo
Language:Inglês
Published: Cold Spring Harbor Laboratory Press 2003
Subjects:
Methods
Online Access:https://ncbi.nlm.nih.gov/pmc/articles/PMC430925/
https://ncbi.nlm.nih.gov/pubmed/12727911
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1101/gr.861503
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