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Dissociation and reassociation of immobilized porphobilinogen synthase: use of immobilized subunits for enzyme isolation.

The dissociation and association of an immobilized preparation of the octameric enzyme porphobilinogen synthase [5-aminolevulinate hydro-lyase (adding 5-aminolevulinate and cyclizing), EC 4.2.1.24] is described. On treatment of the immobilized preparation with 4 M urea, four subunits per octamer are...

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Publicat a:Proc Natl Acad Sci U S A
Autors principals: Gurne, D, Chen, J, Shemin, D
Format: Artigo
Idioma:Inglês
Publicat: National Academy of Sciences 1977
Matèries:
Accés en línia:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC430767/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/266180/
https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.74.4.1383
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