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Isolation of inverted repeat sequences, including IS1, IS2, and IS3, in Escherichia coli plasmids.
A method is described for isolation of inverted repeat DNA sequences that occur in E. coli plasmids. The procedures of the isolation involved: (a) denaturation of intact plasmid DNA, (b) a rapid, 30 sec, renaturation of inverted-repeat sequences in the genome, (c) digestion of the single-stranded po...
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| Publicado no: | Proc Natl Acad Sci U S A |
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| Main Authors: | , |
| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
National Academy of Sciences
1976
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC430545/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/781675/ https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.73.7.2316 |
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