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Isolation of inverted repeat sequences, including IS1, IS2, and IS3, in Escherichia coli plasmids.

A method is described for isolation of inverted repeat DNA sequences that occur in E. coli plasmids. The procedures of the isolation involved: (a) denaturation of intact plasmid DNA, (b) a rapid, 30 sec, renaturation of inverted-repeat sequences in the genome, (c) digestion of the single-stranded po...

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Hlavní autoři: Ohtsubo, H, Ohtsubo, E
Médium: Artigo
Jazyk:Inglês
Vydáno: 1976
Témata:
On-line přístup:https://ncbi.nlm.nih.gov/pmc/articles/PMC430545/
https://ncbi.nlm.nih.gov/pubmed/781675
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