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Tryptic peptide analysis of normal and mutant forms of hypoxanthine phosphoribosyltransferase from HeLa cells.

Hypoxanthine phosphoribosyltransferase (HPRT, IMP:pyrophosphate phosphoribosyltransferase, EC 2.4.2.8) can be purified 5-to 10,000-fold from extracts of HeLa (human) cells by a three-step procedure consisting of high-speed centrifugation, adsorption to Sepharose-conjugated HPRT antibody, and sodium...

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Autors principals: Milman, G, Krauss, S W, Olsen, A S
Format: Artigo
Idioma:Inglês
Publicat: 1977
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Accés en línia:https://ncbi.nlm.nih.gov/pmc/articles/PMC430534/
https://ncbi.nlm.nih.gov/pubmed/265586
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