Analysis of HeLa cell hypoxanthine phosphoribosyltransferase mutants and revertants by two-dimensional polyacrylamide gel electrophoresis: evidence for silent gene activation.

The spot corresponding to hypoxanthine phosphoribosyltransferase (HPRT; IMP:pyrophosphate phosphoribosyltransferase, EC 2.4.2.8) has been identified in two-dimensional polyacrylamide gels of HeLa cell extracts. This spot is absent in gels of 24 HPRT dificient mutants. A missense mutant displays a ne...

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Detalhes bibliográficos
Main Authors: Milman, G, Lee, E, Ghangas, G S, McLaughlin, J R, George, M
Formato: Artigo
Idioma:Inglês
Publicado em: 1976
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Research Article
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC431556/
https://ncbi.nlm.nih.gov/pubmed/63948
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