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Expression and crystallization of a bacterial glycoside hydrolase family 116 β-glucosidase from Thermoanaerobacterium xylanolyticum

The Thermoanaerobacterium xylanolyticum gene product TxGH116, a glycoside hydrolase family 116 protein of 806 amino-acid residues sharing 37% amino-acid sequence identity over 783 residues with human glucosylceramidase 2 (GBA2), was expressed in Escherichia coli. Purification by heating, immobilized...

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Bibliografiske detaljer
Udgivet i:Acta Crystallogr F Struct Biol Commun
Main Authors: Sansenya, Sompong, Mutoh, Risa, Charoenwattanasatien, Ratana, Kurisu, Genji, Ketudat Cairns, James R.
Format: Artigo
Sprog:Inglês
Udgivet: International Union of Crystallography 2015
Fag:
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC4304745/
https://ncbi.nlm.nih.gov/pubmed/25615966
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1107/S2053230X14025461
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