Chromosomal Deletion Formation System Based on Tn5 Double Transposition: Use For Making Minimal Genomes and Essential Gene Analysis

In this communication, we describe the use of specialized transposons (Tn5 derivatives) to create deletions in the Escherichia coli K-12 chromosome. These transposons are essentially rearranged composite transposons that have been assembled to promote the use of the internal transposon ends, resulti...

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Bibliografiske detaljer
Main Authors: Goryshin, Igor Y., Naumann, Todd A., Apodaca, Jennifer, Reznikoff, William S.
Format: Artigo
Sprog:Inglês
Udgivet: Cold Spring Harbor Laboratory Press 2003
Fag:
Methods
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC430159/
https://ncbi.nlm.nih.gov/pubmed/12654720
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1101/gr.611403
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