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Genome engineering for improved recombinant protein expression in Escherichia coli

A metabolic engineering perspective which views recombinant protein expression as a multistep pathway allows us to move beyond vector design and identify the downstream rate limiting steps in expression. In E.coli these are typically at the translational level and the supply of precursors in the for...

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Detalhes bibliográficos
Publicado no:Microb Cell Fact
Main Authors: Mahalik, Shubhashree, Sharma, Ashish K, Mukherjee, Krishna J
Formato: Artigo
Idioma:Inglês
Publicado em: BioMed Central 2014
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC4300154/
https://ncbi.nlm.nih.gov/pubmed/25523647
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1186/s12934-014-0177-1
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