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A novel knock out strategy to enhance recombinant protein expression in Escherichia coli

BACKGROUND: The expression of recombinant proteins triggers a stress response which downregulates key metabolic pathway genes leading to a decline in cellular health and feedback inhibition of both growth and protein expression. Instead of individually upregulating these downregulated genes or impro...

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Bibliografiske detaljer
Udgivet i:Microb Cell Fact
Main Authors: Sharma, Ashish K., Shukla, Esha, Janoti, Deepak S., Mukherjee, Krishna J., Shiloach, Joseph
Format: Artigo
Sprog:Inglês
Udgivet: BioMed Central 2020
Fag:
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC7376861/
https://ncbi.nlm.nih.gov/pubmed/32703203
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1186/s12934-020-01407-z
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