Kinetic pathway of 40S ribosomal subunit recruitment to hepatitis C virus internal ribosome entry site

Translation initiation can occur by multiple pathways. To delineate these pathways by single-molecule methods, fluorescently labeled ribosomal subunits are required. Here, we labeled human 40S ribosomal subunits with a fluorescent SNAP-tag at ribosomal protein eS25 (RPS25). The resulting ribosomal s...

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Bibliografiske detaljer
Udgivet i:Proc Natl Acad Sci U S A
Main Authors: Fuchs, Gabriele, Petrov, Alexey N., Marceau, Caleb D., Popov, Lauren M., Chen, Jin, O’Leary, Seán E., Wang, Richard, Carette, Jan E., Sarnow, Peter, Puglisi, Joseph D.
Format: Artigo
Sprog:Inglês
Udgivet: National Academy of Sciences 2015
Fag:
Biological Sciences
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC4299178/
https://ncbi.nlm.nih.gov/pubmed/25516984
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1073/pnas.1421328111
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