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Two steps forward, one step back: determining XPD helicase mechanism by single-molecule fluorescence and high-resolution optical tweezers

XPD-like helicases constitute a prominent DNA helicase family critical for many aspects of genome maintenance. These enzymes share a unique structural feature, an auxiliary domain stabilized by an iron-sulphur (FeS) cluster, and a 5′-3′ polarity of DNA translocation and duplex unwinding. Biochemical...

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Détails bibliographiques
Publié dans:DNA Repair (Amst)
Auteur principal: Spies, Maria
Format: Artigo
Langue:Inglês
Publié: 2014
Sujets:
Accès en ligne:https://ncbi.nlm.nih.gov/pmc/articles/PMC4295835/
https://ncbi.nlm.nih.gov/pubmed/24560558
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.dnarep.2014.01.013
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