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Click chemistry facilitates direct labelling and super-resolution imaging of nucleic acids and proteins

We demonstrate high-density labelling of cellular DNA and RNA using click chemistry and perform confocal and super-resolution microscopy. We visualize the crescent and ring-like structure of densely packed RNA in nucleoli. We further demonstrate click chemistry with unnatural amino acids for super-r...

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Dettagli Bibliografici
Pubblicato in:RSC Adv
Autori principali: Raulf, Anika, Spahn, Christoph K., Zessin, Patrick J. M., Finan, Kieran, Bernhardt, Stefan, Heckel, Alexander, Heilemann, Mike
Natura: Artigo
Lingua:Inglês
Pubblicazione: Royal Society of Chemistry 2014
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Accesso online:https://ncbi.nlm.nih.gov/pmc/articles/PMC4285124/
https://ncbi.nlm.nih.gov/pubmed/25580242
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1039/c4ra01027b
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