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Bridging of a substrate between cyclodextrin and an enzyme’s active site pocket triggers a unique mode of inhibition

BACKGROUND: Methionyl-7-amino-4-methylcoumarin (MetAMC) serves as a substrate for the E. coli Methionine aminopeptidase (MetAP) catalyzed reaction, and is routinely used for screening compounds to identify potential antibiotic agents. In pursuit of screening the enzyme’s inhibitors, we observed that...

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Bibliografiske detaljer
Udgivet i:Biochim Biophys Acta
Main Authors: Sule, Nitesh V, Ugrinov, Angel, Mallik, Sanku, Srivastava, D. K.
Format: Artigo
Sprog:Inglês
Udgivet: 2014
Fag:
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC4281286/
https://ncbi.nlm.nih.gov/pubmed/25450177
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.bbagen.2014.10.016
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