Sequence-Specific Cleavage of Small-Subunit (SSU) rRNA with Oligonucleotides and RNase H: a Rapid and Simple Approach to SSU rRNA-Based Quantitative Detection of Microorganisms

A rapid and simple approach to the small-subunit (SSU) rRNA-based quantitative detection of a specific group of microorganisms in complex ecosystems has been developed. The method employs sequence-specific cleavage of rRNA molecules with oligonucleotides and RNase H. Defined mixtures of SSU rRNAs we...

Descrición completa

Gardado en:
Detalles Bibliográficos
Main Authors: Uyeno, Yutaka, Sekiguchi, Yuji, Sunaga, Akiko, Yoshida, Hiroki, Kamagata, Yoichi
Formato: Artigo
Idioma:Inglês
Publicado: American Society for Microbiology 2004
Assuntos:
Methods
Acceso en liña:https://ncbi.nlm.nih.gov/pmc/articles/PMC427779/
https://ncbi.nlm.nih.gov/pubmed/15184170
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/AEM.70.6.3650-3663.2004
Tags: Engadir etiqueta
Sen Etiquetas, Sexa o primeiro en etiquetar este rexistro!

Títulos similares