Sequence-Specific Cleavage of Small-Subunit (SSU) rRNA with Oligonucleotides and RNase H: a Rapid and Simple Approach to SSU rRNA-Based Quantitative Detection of Microorganisms

A rapid and simple approach to the small-subunit (SSU) rRNA-based quantitative detection of a specific group of microorganisms in complex ecosystems has been developed. The method employs sequence-specific cleavage of rRNA molecules with oligonucleotides and RNase H. Defined mixtures of SSU rRNAs we...

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Autores principales: Uyeno, Yutaka, Sekiguchi, Yuji, Sunaga, Akiko, Yoshida, Hiroki, Kamagata, Yoichi
Formato: Artigo
Lenguaje:Inglês
Publicado: American Society for Microbiology 2004
Materias:
Methods
Acceso en línea:https://ncbi.nlm.nih.gov/pmc/articles/PMC427779/
https://ncbi.nlm.nih.gov/pubmed/15184170
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/AEM.70.6.3650-3663.2004
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