Highly Efficient Large-Scale Lentiviral Vector Concentration by Tandem Tangential Flow Filtration

Large-scale lentiviral vector (LV) concentration can be inefficient and time consuming, often involving multiple rounds of filtration and centrifugation. This report describes a simpler method using two tangential flow filtration (TFF) steps to concentrate liter-scale volumes of LV supernatant, achi...

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Bibliographic Details
Published in:J Virol Methods
Main Authors: Cooper, Aaron R., Patel, Sanjeet, Senadheera, Shantha, Plath, Kathrin, Kohn, Donald B., Hollis, Roger P.
Format: Artigo
Language:Inglês
Published: 2011
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Article
Online Access:https://ncbi.nlm.nih.gov/pmc/articles/PMC4276401/
https://ncbi.nlm.nih.gov/pubmed/21784103
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.jviromet.2011.06.019
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