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Asymmetric parental genome engineering by Cas9 during mouse meiotic exit

Mammalian genomes can be edited by injecting pronuclear embryos with Cas9 cRNA and guide RNA (gRNA) but it is unknown whether editing can also occur during the onset of embryonic development, prior to pronuclear embryogenesis. We here report Cas9-mediated editing during sperm-induced meiotic exit an...

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書目詳細資料
發表在:Sci Rep
Main Authors: Suzuki, Toru, Asami, Maki, Perry, Anthony C. F.
格式: Artigo
語言:Inglês
出版: Nature Publishing Group 2014
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在線閱讀:https://ncbi.nlm.nih.gov/pmc/articles/PMC4274505/
https://ncbi.nlm.nih.gov/pubmed/25532495
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/srep07621
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