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Asymmetric parental genome engineering by Cas9 during mouse meiotic exit

Mammalian genomes can be edited by injecting pronuclear embryos with Cas9 cRNA and guide RNA (gRNA) but it is unknown whether editing can also occur during the onset of embryonic development, prior to pronuclear embryogenesis. We here report Cas9-mediated editing during sperm-induced meiotic exit an...

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Detalhes bibliográficos
Publicado no:Sci Rep
Main Authors: Suzuki, Toru, Asami, Maki, Perry, Anthony C. F.
Formato: Artigo
Idioma:Inglês
Publicado em: Nature Publishing Group 2014
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC4274505/
https://ncbi.nlm.nih.gov/pubmed/25532495
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/srep07621
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