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Recoding Aminoacyl-tRNA Synthetases for Synthetic Biology by Rational Protein-RNA Engineering
[Image: see text] We have taken a rational approach to redesigning the amino acid binding and aminoacyl–tRNA pairing specificities of bacterial glutaminyl–tRNA synthetase. The four-stage engineering incorporates generalizable design principles and improves the pairing efficiency of noncognate glutam...
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| Veröffentlicht in: | ACS Chem Biol |
|---|---|
| Hauptverfasser: | , |
| Format: | Artigo |
| Sprache: | Inglês |
| Veröffentlicht: |
American Chemical
Society
2014
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| Online Zugang: | https://ncbi.nlm.nih.gov/pmc/articles/PMC4273986/ https://ncbi.nlm.nih.gov/pubmed/25310879 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1021/cb5006596 |
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