Use of Adaptive Laboratory Evolution To Discover Key Mutations Enabling Rapid Growth of Escherichia coli K-12 MG1655 on Glucose Minimal Medium

Adaptive laboratory evolution (ALE) has emerged as an effective tool for scientific discovery and addressing biotechnological needs. Much of ALE's utility is derived from reproducibly obtained fitness increases. Identifying causal genetic changes and their combinatorial effects is challenging a...

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Detalhes bibliográficos
Publicado no:Appl Environ Microbiol
Main Authors: LaCroix, Ryan A., Sandberg, Troy E., O'Brien, Edward J., Utrilla, Jose, Ebrahim, Ali, Guzman, Gabriela I., Szubin, Richard, Palsson, Bernhard O., Feist, Adam M.
Formato: Artigo
Idioma:Inglês
Publicado em: American Society for Microbiology 2014
Assuntos:
Evolutionary and Genomic Microbiology
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC4272732/
https://ncbi.nlm.nih.gov/pubmed/25304508
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/AEM.02246-14
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