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Spectral Properties of an Oxygenated Luciferase—Flavin Intermediate Isolated by Low-Temperature Chromatography
Bacterial luciferase catalyzes the oxidation of reduced flavin mononucleotide by molecular oxygen; long-chain aldehyde is required for light emission. At 20° the bioluminescence has a lifetime of tens of seconds, while excess reduced flavin is removed by way of nonenzymatic autoxidation in less than...
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| Publicado no: | Proc Natl Acad Sci U S A |
|---|---|
| Main Authors: | , , , |
| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
National Academy of Sciences
1973
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC427261/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/16592121/ https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.70.12.3468 |
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