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Spectral Properties of an Oxygenated Luciferase—Flavin Intermediate Isolated by Low-Temperature Chromatography

Bacterial luciferase catalyzes the oxidation of reduced flavin mononucleotide by molecular oxygen; long-chain aldehyde is required for light emission. At 20° the bioluminescence has a lifetime of tens of seconds, while excess reduced flavin is removed by way of nonenzymatic autoxidation in less than...

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Pubblicato in:Proc Natl Acad Sci U S A
Autori principali: Hastings, J. W., Balny, Claude, Peuch, Christian Le, Douzou, Pierre
Natura: Artigo
Lingua:Inglês
Pubblicazione: National Academy of Sciences 1973
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Accesso online:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC427261/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/16592121/
https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.70.12.3468
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