Microfluorometric Detection of Deoxyribonucleic Acid Replication in Human Metaphase Chromosomes
Fluorescence of the dye 33258 Hoechst bound to chromosomes is partially quenched by the introduction of BrdU into chromosomal DNA. This finding has allowed microfluorometric detection of DNA synthesis in human metaphase chromosomes. Incorporation, shortly before cell harvest, of a pulse of thymidine...
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| Pubblicato in: | Proc Natl Acad Sci U S A |
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| Autore principale: | |
| Natura: | Artigo |
| Lingua: | Inglês |
| Pubblicazione: |
National Academy of Sciences
1973
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| Soggetti: | |
| Accesso online: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC427244/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/4128545/ https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.70.12.3395 |
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