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Relation of Detergent HLB Number to Solubilization and Stabilization of D-Alanine Carboxypeptidase from Bacillus subtilis Membranes

The ability of various nonionic detergents to solubilize D-alanine carboxypeptidase and other membrane-bound enzymes was correlated to a physical property of the detergent, the HLB number. Only a fairly narrow range of detergents were effective solubilizing agents. Purified carboxypeptidase required...

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Pubblicato in:Proc Natl Acad Sci U S A
Autori principali: Umbreit, Jay N., Strominger, Jack L.
Natura: Artigo
Lingua:Inglês
Pubblicazione: National Academy of Sciences 1973
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Accesso online:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC427155/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/4200727/
https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.70.10.2997
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