Isolation of Supercoiled Colicinogenic Factor E(1) DNA Sensitive to Ribonuclease and Alkali

The synthesis of the covalently-closed, circular DNA form of colicinogenic factor E(1) (ColE(1)) continues in Escherichia coli cells after the addition of chloramphenicol. A large portion of the purified supercoiled ColE(1) DNA molecules made in the presence of chloramphenicol are converted to the o...

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Detalhes bibliográficos
Publicado no:Proc Natl Acad Sci U S A
Main Authors: Blair, D. G., Sherratt, D. J., Clewell, D. B., Helinski, D. R.
Formato: Artigo
Idioma:Inglês
Publicado em: National Academy of Sciences 1972
Assuntos:
Biological Sciences: Biochemistry
Acesso em linha:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC426978/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/4560690/
https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.69.9.2518
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