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Identification and characterization of CbeI, a novel thermostable restriction enzyme from Caldicellulosiruptor bescii DSM 6725 and a member of a new subfamily of HaeIII-like enzymes

Potent HaeIII-like DNA restriction activity was detected in cell-free extracts of Caldicellulosiruptor bescii DSM 6725 using plasmid DNA isolated from Escherichia coli as substrate. Incubation of the plasmid DNA in vitro with HaeIII methyltransferase protected it from cleavage by HaeIII nuclease as...

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Bibliografski detalji
Izdano u:J Ind Microbiol Biotechnol
Glavni autori: Chung, Dae-Hwan, Huddleston, Jennifer R., Farkas, Joel, Westpheling, Janet
Format: Artigo
Jezik:Inglês
Izdano: 2011
Teme:
Online pristup:https://ncbi.nlm.nih.gov/pmc/articles/PMC4269323/
https://ncbi.nlm.nih.gov/pubmed/21604181
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1007/s10295-011-0976-x
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