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Effect of Environmental pH on the Efficiency of Cellular Hybridization

The hybridization of a human and mouse cell was strikingly pH-dependent, with a well defined optimum at (about) pH 7.6-8.0. The yield of hybrid cell colonies (1 per 500-2000 heterokaryocytes) was several hundred times greater than that obtained at pH 6.8-7.2. Although there was a significant effect...

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Библиографические подробности
Опубликовано в: :Proc Natl Acad Sci U S A
Главные авторы: Croce, Carlo M., Koprowski, Hilary, Eagle, Harry
Формат: Artigo
Язык:Inglês
Опубликовано: National Academy of Sciences 1972
Предметы:
Online-ссылка:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC426839/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/4340167/
https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.69.7.1953
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