Effect of Environmental pH on the Efficiency of Cellular Hybridization
The hybridization of a human and mouse cell was strikingly pH-dependent, with a well defined optimum at (about) pH 7.6-8.0. The yield of hybrid cell colonies (1 per 500-2000 heterokaryocytes) was several hundred times greater than that obtained at pH 6.8-7.2. Although there was a significant effect...
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| Published in: | Proc Natl Acad Sci U S A |
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| Main Authors: | , , |
| Format: | Artigo |
| Language: | Inglês |
| Published: |
National Academy of Sciences
1972
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| Subjects: | |
| Online Access: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC426839/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/4340167/ https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.69.7.1953 |
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