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Increased Efficiency of Exogenous Messenger RNA Translation in a Krebs Ascites Cell Lysate

Addition of a 0.5 M KCl wash fraction from rabbit reticulocyte ribosomes causes a 3- to 10-fold increase in the extent of translation of natural mRNAs by Krebs-cell lysates. In the presence of the wash fraction, 1 pmol of rabbit or mouse 10S RNA directs the incorporation of 80 pmol of leucine into r...

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Bibliographic Details
Main Authors: Metafora, S., Terada, M., Dow, L. W., Marks, P. A., Bank, A.
Format: Artigo
Language:Inglês
Published: 1972
Subjects:
Online Access:https://ncbi.nlm.nih.gov/pmc/articles/PMC426686/
https://ncbi.nlm.nih.gov/pubmed/4338590
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