Affinity Labeling of the Acetylcholine Receptor in the Electroplax: Electrophoretic Separation in Sodium Dodecyl Sulfate
Electroplax, single cells dissected from electric tissue of Electrophorus, are labeled in a two-step procedure: reduction by dithiothreitol followed by alkylation by the affinity label 4-(N-maleimido)-α-benzyltri-[methyl-(3)H]methylammonium iodide, either alone or in combination with [2,3-(14)C]N-et...
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| Udgivet i: | Proc Natl Acad Sci U S A |
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| Principais autores: | , , , |
| Format: | Artigo |
| Sprog: | Inglês |
| Udgivet: |
National Academy of Sciences
1972
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| Fag: | |
| Online adgang: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC426655/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/4504331/ https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.69.5.1168 |
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