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Affinity Labeling of the Acetylcholine Receptor in the Electroplax: Electrophoretic Separation in Sodium Dodecyl Sulfate

Electroplax, single cells dissected from electric tissue of Electrophorus, are labeled in a two-step procedure: reduction by dithiothreitol followed by alkylation by the affinity label 4-(N-maleimido)-α-benzyltri-[methyl-(3)H]methylammonium iodide, either alone or in combination with [2,3-(14)C]N-et...

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Detalles Bibliográficos
Main Authors: Reiter, Michael J., Cowburn, David A., Prives, Joav M., Karlin, Arthur
Formato: Artigo
Idioma:Inglês
Publicado: 1972
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Acceso en liña:https://ncbi.nlm.nih.gov/pmc/articles/PMC426655/
https://ncbi.nlm.nih.gov/pubmed/4504331
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