Protein interference applications in cellular and developmental biology using DARPins that recognize GFP and mCherry

Protein–protein interactions are crucial for cellular homeostasis and play important roles in the dynamic execution of biological processes. While antibodies represent a well-established tool to study protein interactions of extracellular domains and secreted proteins, as well as in fixed and permea...

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Bibliografiske detaljer
Udgivet i:Biol Open
Main Authors: Brauchle, Michael, Hansen, Simon, Caussinus, Emmanuel, Lenard, Anna, Ochoa-Espinosa, Amanda, Scholz, Oliver, Sprecher, Simon G., Plückthun, Andreas, Affolter, Markus
Format: Artigo
Sprog:Inglês
Udgivet: The Company of Biologists 2014
Fag:
Methods & Techniques
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC4265764/
https://ncbi.nlm.nih.gov/pubmed/25416061
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1242/bio.201410041
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