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Using baculovirus/insect cell expressed recombinant actin to study the molecular pathogenesis of HCM caused by actin mutation A331P

Recombinant WT human cardiac actin (WT actin) was expressed using the baculovirus/insect cell expression system, purified, and used to reconstitute the thin-filament of bovine cardiac muscle fibers, together with bovine cardiac tropomyosin (Tm) and troponin (Tn). Effects of [Ca(2+)], [ATP], [phospha...

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Detalhes bibliográficos
Publicado no:J Mol Cell Cardiol
Main Authors: Bai, Fan, Caster, Hannah M., Rubenstein, Peter A., Dawson, John F., Kawai, Masataka
Formato: Artigo
Idioma:Inglês
Publicado em: 2014
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC4264970/
https://ncbi.nlm.nih.gov/pubmed/24793351
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.yjmcc.2014.04.014
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