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Insulin Biosynthesis: Studies of Islet Polyribosomes

A method is described for separation of polyribosomes from as few as 25 isolated Islets of Langerhans, representing about 250 μg of pancreatic tissue. Islets are labeled with [(3)H]leucine and polysomes are isolated with liver polyribosomes, which serve as carrier and inhibitor of ribonuclease activ...

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Detalhes bibliográficos
Publicado no:Proc Natl Acad Sci U S A
Main Authors: Permutt, M. A., Kipnis, D. M.
Formato: Artigo
Idioma:Inglês
Publicado em: National Academy of Sciences 1972
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC426490/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/4551147/
https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.69.2.505
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