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Directed hydroxyl radical probing of 16S rRNA using Fe(II) tethered to ribosomal protein S4.

Localized hydroxyl radical probing has been used to explore the rRNA neighborhood around a unique position in the structure of the Escherichia coli 30S ribosomal subunit. Fe(II) was attached to ribosomal protein S4 at Cys-31 via the reagent 1-(p-bromoacetamidobenzyl)-EDTA. [Fe-Cys31]S4 was then comp...

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Autors principals: Heilek, G M, Marusak, R, Meares, C F, Noller, H F
Format: Artigo
Idioma:Inglês
Publicat: 1995
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Accés en línia:https://ncbi.nlm.nih.gov/pmc/articles/PMC42648/
https://ncbi.nlm.nih.gov/pubmed/7862644
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