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Directed hydroxyl radical probing of 16S rRNA in the ribosome: spatial proximity of RNA elements of the 3' and 5' domains.

We have shown previously that directed hydroxyl radical probing of 16S rRNA from Fe(II) tethered to specific sites within the RNA gives valuable information about RNA-RNA proximities in 70S ribosomes. Here, we extend that study and present probing data from nt 424 in 16S rRNA. To tether an Fe(II) to...

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Autors principals: Newcomb, L F, Noller, H F
Format: Artigo
Idioma:Inglês
Publicat: 1999
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Accés en línia:https://ncbi.nlm.nih.gov/pmc/articles/PMC1369809/
https://ncbi.nlm.nih.gov/pubmed/10411128
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