The location of protein S8 and surrounding elements of 16S rRNA in the 70S ribosome from combined use of directed hydroxyl radical probing and X-ray crystallography.

Ribosomal protein S8, which is essential for the assembly of the central domain of 16S rRNA, is one of the most thoroughly studied RNA-binding proteins. To map its surrounding RNA in the ribosome, we carried out directed hydroxyl radical probing of 16S rRNA using Fe(II) tethered to nine different po...

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Hauptverfasser: Lancaster, L, Culver, G M, Yusupova, G Z, Cate, J H, Yusupov, M M, Noller, H F
Format: Artigo
Sprache:Inglês
Veröffentlicht: 2000
Schlagworte:
Research Article
Online Zugang:https://ncbi.nlm.nih.gov/pmc/articles/PMC1369952/
https://ncbi.nlm.nih.gov/pubmed/10836793
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