A New Micromethod for Evaluating Lymphocyte Responses to Phytohemagglutinin: Quantitative Analysis of the Function of Thymus-Dependent Cells
We have developed a new micromethod for testing phytohemagglutinin stimulation of human lymphocytes. 50 μl of peripheral blood is incubated at 37°C directly, without cell separation, with 50 μl of tissue culture medium containing 50μg of phytohemagglutinin for 24 hr. Then, 0.5 μCi of tritiated thymi...
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| Udgivet i: | Proc Natl Acad Sci U S A |
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| Principais autores: | , |
| Format: | Artigo |
| Sprog: | Inglês |
| Udgivet: |
National Academy of Sciences
1972
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| Fag: | |
| Online adgang: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC426460/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/4501119/ https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.69.2.371 |
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