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A New Micromethod for Evaluating Lymphocyte Responses to Phytohemagglutinin: Quantitative Analysis of the Function of Thymus-Dependent Cells
We have developed a new micromethod for testing phytohemagglutinin stimulation of human lymphocytes. 50 μl of peripheral blood is incubated at 37°C directly, without cell separation, with 50 μl of tissue culture medium containing 50μg of phytohemagglutinin for 24 hr. Then, 0.5 μCi of tritiated thymi...
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| Main Authors: | , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado: |
1972
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| Assuntos: | |
| Acceso en liña: | https://ncbi.nlm.nih.gov/pmc/articles/PMC426460/ https://ncbi.nlm.nih.gov/pubmed/4501119 |
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