Determination of absolute amounts of GDP and GTP bound to Ras in mammalian cells: comparison of parental and Ras-overproducing NIH 3T3 fibroblasts.

We devised enzyme-based methods to measure fmol amounts of GDP and GTP and applied these methods to measure absolute amounts of Ras-bound GDP and GTP in NIH 3T3 fibroblasts. We found that parental NIH 3T3 cells contained 509 and 1.3 fmol of Ras-bound GDP and GTP, respectively, per mg of cellular pro...

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Bibliografische gegevens

Gepubliceerd in:	Proc Natl Acad Sci U S A
Hoofdauteurs:	Scheele, J S, Rhee, J M, Boss, G R
Formaat:	Artigo
Taal:	Inglês
Gepubliceerd in:	National Academy of Sciences 1995
Onderwerpen:	Research Article
Online toegang:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC42644/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/7862641/ https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.92.4.1097
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