DNA extraction protocols cause differences in 16S rRNA amplicon sequencing efficiency but not in community profile composition or structure

The recent development of methods applying next-generation sequencing to microbial community characterization has led to the proliferation of these studies in a wide variety of sample types. Yet, variation in the physical properties of environmental samples demands that optimal DNA extraction techni...

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Bibliographic Details
Published in:Microbiologyopen
Main Authors: Rubin, Benjamin E R, Sanders, Jon G, Hampton-Marcell, Jarrad, Owens, Sarah M, Gilbert, Jack A, Moreau, Corrie S
Format: Artigo
Language:Inglês
Published: BlackWell Publishing Ltd 2014
Subjects:
Original Research
Online Access:https://ncbi.nlm.nih.gov/pmc/articles/PMC4263514/
https://ncbi.nlm.nih.gov/pubmed/25257543
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1002/mbo3.216
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