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Purification and characterization of recombinant human p50csk protein-tyrosine kinase from an Escherichia coli expression system overproducing the bacterial chaperones GroES and GroEL.

An Escherichia coli expression system overproducing the bacterial chaperones GroES and GroEL was engineered and has been successfully used to produce large quantities of the recombinant human protein-tyrosine kinase p50csk. The co-overproduction of the two chaperones with p50csk results in increased...

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Bibliografiske detaljer
Udgivet i:Proc Natl Acad Sci U S A
Principais autores: Amrein, K E, Takacs, B, Stieger, M, Molnos, J, Flint, N A, Burn, P
Format: Artigo
Sprog:Inglês
Udgivet: National Academy of Sciences 1995
Fag:
Online adgang:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC42634/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/7862631/
https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.92.4.1048
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