A Massively Parallel Pipeline to Clone DNA Variants and Examine Molecular Phenotypes of Human Disease Mutations

Understanding the functional relevance of DNA variants is essential for all exome and genome sequencing projects. However, current mutagenesis cloning protocols require Sanger sequencing, and thus are prohibitively costly and labor-intensive. We describe a massively-parallel site-directed mutagenesi...

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Bibliografiske detaljer
Udgivet i:PLoS Genet
Main Authors: Wei, Xiaomu, Das, Jishnu, Fragoza, Robert, Liang, Jin, Bastos de Oliveira, Francisco M., Lee, Hao Ran, Wang, Xiujuan, Mort, Matthew, Stenson, Peter D., Cooper, David N., Lipkin, Steven M., Smolka, Marcus B., Yu, Haiyuan
Format: Artigo
Sprog:Inglês
Udgivet: Public Library of Science 2014
Fag:
Research Article
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC4263371/
https://ncbi.nlm.nih.gov/pubmed/25502805
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1371/journal.pgen.1004819
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